Orjinal Araştırma Makalesi | Gelecek Vizyonlar Dergisi 2019, Cil. 3(2) 18-22
Melda Şahin & Nurgül Şenol
ss. 18 - 22 | DOI: https://doi.org/10.29345/futvis.70 | Makale No: fvj.2021.034
Yayın tarihi: Aralık 03, 2021 | Okunma Sayısı: 11 | İndirilme Sayısı: 365
Özet
In this study, it was aimed to determine the density of mast cells in the kidney using different fixatives in Goldfinch (Cardueli carduelis). In the present study, 1-2 years three female Goldfinch(Cardueli carduelis) were used. Kidney tissues were included in 10% formaldehyde solution (24-48 hours) and in Bouin solution (16-18 hours). Samples were passed through routine tissue processing and embedded in parafin. Sections (5-6µ) were stained for general morphological purposes with haematoxylin and eosin (H & E) stains. For mast cells identification and density; serial cross section were performed 0.5 % toluidine blue and alcian blue pH 0.3- safranin combination methods. Lastly, preparations were examined under light microscope (Leica DM 500) and appropriate locations were photographed. Mast cells were localized in the medulla, interstitium around renal tubules, cortex, interstitium around glomeruli and the blood vessels . As a result, fixed in Bouin solution of mast cells were showed more clearly metachromatically in pH 0.5 TB applications. In AB / SO combined dye application, mostly blue alcian blue (AB) (+) and less red safranine (SO) (+) cells were detected, while red-blue AB / SO (+) (mixed) mast cells were not detected.
Anahtar Kelimeler: Alcian Blue-Safranin, Fiksatif, Mast Hücresi, Toluidine Blue, Saka
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